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改良Bryant Burkey肉湯

英文名稱：
產品貨號：	HB9249
產品規格：	250g
產品價格：	200元
保質期：	三年
產品用途：	用于青貯飼料、牛奶和乳制品中乳酸發酵梭狀芽孢桿菌孢子的計數
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產品介紹:

Bryant Burkey Broth with Resazurin and Lactate is also known as BB broth and BBMB broth. It??’s used for the enumeration of the spores of lactic acid-fermenting Clostridia spp. in silage, milk and dairy products.

Approximate Formula:

Ingredients	gm/liter
Enzymatic digest of casein	15.0
Meat extract	7.5
Yeast extract	5.0
Cysteine HCl	0.5
Sodium acetate	5.0
Sodium lactate	5.0
Resazurin	0.005
Final pH5.8±0.1	25℃

*Adjusted and/or supplemented ??as required to meet perform??ance criteria.

Directions:

Dissolve 38.0g in 1 liter of purified water and mix well. Dispense ??into tubes or bottles. Autocl?ave at 121℃ for 15 minutes.

According to CNERNA, the medium is dispensed by 10ml into 16x160mm tu?bes before sterilization.

Principle and Interpretation:

Enzymatic digest o??f casein, meat extract, yeast extract and cysteine HCl create the conditions for a rapid growth of lactate fermenting Clostridium spp. Resazurin is a redox indicator and monitors the oxygen level. Sodium lactate acts as the carbon source to allow selective growth of lactate-fermenting spore formers and is the substrate for the gas production of Clostridium spp. Sodium acetate facilitates the lactate fermentation by C. tyrobutyricum. Sodium acetate also promotes the spore germination, which is activated by the heat treatment of the sample.

Appearance:

Dehydrat??ed medium is a free-flo?wing yellowish powder. The prepared medium ??is a kind of yellowish-red turbid liquid.

Precautions:

This medium is for laboratory use only. Dried medium which is past shelf life, caking or col??or variation cannot be used.

Storage conditions and Shelf life:

Bryant Burkey Broth with Resazurin and Lactate must be stored tightly capped in the original container at 5-30°C. The dehydrated medium has a shelf life of 3 years from date of manufac??turing. Prepared medium may be stored, out of direct ?light at 2-8°C.

Quality control:

Pr??epare the medium per label directions. Inoculate and incubate at 36℃ in an anaerobic atmosph?ere for 24-72 hours.

Microorganism	Strains Number	Inoculum (CFU)	Remarks
Clostridium butyricum	ATCC 19398	10-100	Turbid with gas
Clostridium perfringens	ATCC 13124	10-100	Turbid with gas
Clostridium sporogenes	ATCC 19404	10-100	Turbid

Reference:

1. United States Pharmacopeial Convention, Inc. 2008. The United States pharmacopeia 31/The nationalformulary 26, Supp. 1, 8-1-08, online. United States Pharm?acopeial Convention??, Inc., Rockville, Md.

2. European Directorate for the Quality of Medicines and Healthcare. 2008??. The European pharmacopoeia,6th ed., Supp. 1, 4-1-2008, online. European Directorate for the Quality of Medicinesand Healthcare, Council of Europe, 226 Avenue de Colmar BP907-, F-?67029 Strasbourg Cedex 1,France.